For general laboratory use. Others EvoScript Universal cDNA Master GLU EvoScript Universal cDNA Master 3.6.8.40.1.2 EvoScript Universal cDNA Master 04038377029801 1 kit EvoScript Universal cDNA Master 100 rxn 07912439001 up to 100 reactions of 20 µl final volume each Reagents, kits EvoScript Universal cDNA Master 04038377029818 1 kit EvoScript Universal cDNA Master 200 rxn 07912455001 up to 200 reactions of 20 µl final volume each Reagents, kits EvoScript Universal cDNA Master 04038377029795 1 kit EvoScript Universal cDNA Master 50 rxn 07912374001 up to 50 reactions of 20 µl final volume each Reagents, kits en The EvoScript Universal cDNA Master is designed for highly sensitive and convenient cDNA synthesis for use in two-step RT-qPCR and is compatible with the LightCycler® 480 System, LightCycler® 96 System, or other real-time PCR instruments. The 2-vial composition is ideally suited for reverse transcription, requiring only the addition of target RNA. en The EvoScript Universal cDNA Master provides a convenient solution for time-saving cDNA Synthesis for use in two-step, real-time RT-qPCR. All reagents needed for cDNA synthesis, including primers, nucleotides, buffers, and enzymes are supplied in only two vials, minimizing pipetting efforts. The enzyme blend included in this kit has a broad temperature range and is suitable for high temperature reverse transcription. The amount of random primer and oligo(dT)18 primer included in the EvoScript Universal cDNA Master Reaction Buffer enables high cDNA yields from all regions of the RNA template. The product is tested for purified cellular RNA, mRNA, and cell lysates generated using the RealTime ready Cell Lysis Buffer* which further accelerates fast real-time PCR directly on cell lysates. If large amounts of cDNA are required, the reaction can be upscaled without influencing the product yield.The EvoScript Universal cDNA Master enables reliable cDNA synthesis over a wide dynamic range even for GC-rich templates, and is ideal for high-throughput quantitative RT-PCR analysis. The mix contains an optimized concentration of Mg(OAc)2, eliminating the need for additional adjustments.